CeSPIAStructural analysis business
(New drug development strategy: Including drug rescuing)・Conclude a secret contract, etc., and conduct research and testing of commissioned samples in accordance with the content of the requests.・Conclude a contract based on the results of studies such as the prospects for construction of the expression system, purification, and structural analysis.
Example of a contract
1) Everything from the expression of target protein to its structural analysis is carried out at CeSPIA (necessary new technology development is also carried out).2) Conduct everything from the expression of target protein to its structural analysis, but accept the dispatch of human resources from the consignor (considering the reduction of analysis fees and technology transfer), and conduct structural analysis at CeSPIA.3) Preparation of purified samples is carried out on the consignor's side, and only structural analysis is performed by CeSPIA.4) New drug discovery strategies based on structural information: Challenge such as drug rescuing (support for various types of contracts such as joint research)
Drug discovery through new drug discovery strategies, etc.
・Drug discovery strategy based on the results of structural and functional studies・Drug candidate developed by SGDD will be produced・New drug discovery strategy based on drug rescuing・Testing services for neurological diseases・Services and consulting related to the above
Business Services
We will present our services in structural analysis of proteins and macromolecular complexes by single particle method using cryo-electron microscopy. Structure-guided drug development (SGDD) techniques are also being improved and we will develop and propose a new drug candidate based on SGDD.
Specific Services and Technical Content
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Expression and purification of target proteins
・Sf9, Sf+ and BacMam systems and gene design and synthesis・Rapid expression check, purification check, and actual large scale expression and purification -
Preparation of samples for electron microscopy with atomic resolution in mind
・Technology to fabricate an EM grid with the smallest amount of sample without changing the particle distribution・Preparation of ice-embedded samples with optimal thickness・EM grid fabrication technology that is as random as possible in particle orientation -
High-resolution low-temperature electron microscope system with direct electron detector K3 camera
・Technology for embedding particles in thin ice of optimal thickness, which is necessary for high-resolution analysis・High-resolution low-temperature electron microscope system for stable and quick sample exchange・High-efficiency system that can simultaneously view the field of view mode and the actual high-resolution image・Large-capacity recording system that can store images with motion correction at high speed -
A computer system capable of processing a huge amount of data, which is essential for single-particle analysis
・Technology for high-speed, high-resolution analysis of hundreds of thousands of particle images・Cluster computer system capable of performing enormous calculations・Knowledge and experience to correctly analyze and construct structural models
